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高效毛细管电泳(HPCE)是以高电场为驱动力,在细内径毛细管内带电荷粒子按其淌度或分配系数的不同而进行分离的一种电泳新技术。由于其分离效能高,分析速度快,样品用量少,在中药成份分析~([1])、抗生素~([2])、生物大分子~([3])等方面应用较广,近来在体内药物分析的应用逐渐增多。1基本原理HPCE主要的分离模式~([4])分为毛细管区带电泳(CZE)、胶束电动毛细管电泳(MECC)、毛细管凝胶电泳(CGE)、毛细管等电聚焦(CIEF)、毛细管等速电泳(CITP),而CZE和
High-performance capillary electrophoresis (HPCE) is a high-field-driven, small-diameter capillary in the charged particles according to their mobility or partition coefficient of the separation of a new electrophoresis technology. Due to its high separation efficiency, high speed of analysis and low sample usage, it has been widely used in the analysis of Chinese medicine components (1), antibiotics (2) and biological macromolecules (3) The application of in vivo drug analysis is gradually increasing. 1 Principle The main separation mode of HPCE is divided into capillary zone electrophoresis (CZE), micellar electrokinetic capillary electrophoresis (MECC), capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary Constant-rate electrophoresis (CITP), while CZE and