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植物名称:牡丹(Paeonia Suffruticosa)。材料类别:成熟种子经清水浸泡一周,取出放入饱和的漂白粉上清液中浸泡10分钟,再用0.1％升汞溶液浸泡5分钟,无菌水冲洗干净,在超净工作台上剖开种子取胚接种。培养条件:在胚培养的初始生长发育和丛生芽的诱导产生等不同阶段分别采用以下不同组的培养基。A、MS+BA0.5(mg/l,下同)+IAAI+蔗糖3％
Plant name: Paeonia Suffruticosa. Material category: mature seeds soaked in water for one week, remove the saturated bleach supernatant soak for 10 minutes, and then soaked in 0.1% Mercury solution for 5 minutes, rinse with sterile water, cut open the seeds in the clean bench Take the embryo inoculation. Culture conditions: The following different groups of media were used at different stages of initial growth and development of embryo culture and induction of clustered shoots, respectively. A, MS + BA0.5 (mg / l, the same below) + IAAI + sucrose 3%