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目的研究白术茯苓汤不同配比对白术内酯Ⅲ含量的影响。方法采用RP-HPLC法,以Hyperil C18柱(250mm×46mm;5mm)为色谱柱,乙腈-水(48∶52)为流动相,检测波长215nm,流速1mL·min-1,柱温30℃。结果复方中白术内酯Ⅲ0.288～2.016μg的线性良好,回归方程为:Y=3×106X-1.126×105(r=0.9993),平均回收率为98.22%,RSD=1.892%。结论所用方法测定简便快速、可靠,可用于该复方中白术内酯Ⅲ的含量测定。
Objective To study the effect of different combinations of Baizhu Poria Decoction on the content of atractylenolide Ⅲ. Methods The RP-HPLC was performed on a Hyperil C18 column (250 mm × 46 mm; 5 mm) with acetonitrile-water (48:52) as the mobile phase at a flow rate of 1 mL · min-1 at a detection wavelength of 215 nm. Results The linear range of Compound Atractylresin Ⅲ from 0.288 to 2.016 μg was good. The regression equation was Y = 3 × 106X-1.126 × 105 (r = 0.9993). The average recoveries were 98.22% and RSD = 1.892%. Conclusion The method is simple, rapid and reliable and can be used to determine the content of this compound.