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探讨肝硬化患者血清肿瘤坏死因子 (TNFα)、白细胞介素 6 (IL - 6 ) ,透明质酸 (HA)、Ⅳ型胶原 (CⅣ)含量变化及其在肝纤维化中的临床意义。方法 :取空静脉血 ,用放射免疫法测定血清HA、CⅣ含量 ,用双抗体夹心酶联免疫吸附法测定TNFα、IL - 6含量。结果 :肝硬化患者血清TNFα、IL - 6、HA、CⅣ 含量明显高于对照组 (P <0 .0 1) ,且在肝功能分级中呈现ChildC >B >A的规律 ;TNFα、IL - 6与HA、CⅣ 含量呈正相关。结论 :HA、CⅣ 的升高反映肝纤维化程度 ,TNFα、IL - 6参与肝纤维化的形成。
To investigate the changes of serum tumor necrosis factor (TNFα), interleukin - 6 (IL - 6), hyaluronic acid (HA) and collagen Ⅳ (Ⅳ) in patients with liver cirrhosis and their clinical significance in liver fibrosis. Methods: The venous blood samples were collected for determination of serum HA and CⅣ levels by radioimmunoassay. The levels of TNFα and IL - 6 were determined by sandwich enzyme - linked immunosorbent assay. Results: The levels of serum TNFα, IL - 6, HA and C Ⅳ in patients with cirrhosis were significantly higher than those in the control group (P <0.01), and showed the rules of ChildC> B> A in the liver function grading. The levels of TNFα, IL - And HA, CⅣ content was positively correlated. Conclusion: The increase of HA and CⅣ reflects the degree of liver fibrosis, and TNFα and IL - 6 are involved in the formation of hepatic fibrosis.