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目的:分析少毛北前胡氯仿提取部位高效液相色谱法(HPLC)指纹图谱与化痰药效关联性,以确立其化痰“药效成分组”。方法:通过HPLC测定分析少毛北前胡氯仿提取部位HPLC指纹图谱,用小鼠酚红化痰法研究其化痰作用,急支糖浆为阳性对照药(12 mL.kg-1),给小鼠灌胃氯仿提取物6 d(1.4 g.kg-1),用分光光度计测定小鼠气管的酚红分泌量,然后用灰关联度分析方法研究其谱效关系。结果:少毛北前胡氯仿提取部位的化痰作用是其所有化学成分共同作用的结果,各特征峰代表各自化学成分,其对化痰作用贡献的大小顺序(按特征峰编号)为13>12>16>18>19>6>20>14>1>11>15>10>17>2>5>4>7>3>8>9,其中1,3,4,10,13,16号特征峰鉴定为异紫花前胡苷元,补骨脂素,花椒毒素,Pd-Ib,蝉翼素,peuformosin。结论:少毛北前胡氯仿提取部位具有较强化痰活性,其HPLC指纹图谱与化痰作用之间有一定对应关系。
OBJECTIVE: To analyze the correlation between HPLC fingerprinting and phlegm-pharmacodynamics of the chloroform fraction of Shaopian northern forebrain to establish its Huatan group. Methods: The HPLC fingerprints of chloroform extract from Shaohibeiqinqianqiu were analyzed by HPLC. The mice were treated with phlegm-phlegm method to study their chemical constituents of phlegm-resolving effect. Xuezhi syrup was used as positive control (12 mL.kg-1) Rats were intragastrically given chloroform extract for 6 d (1.4 g · kg-1). The phenol red secretion in the trachea of mice was measured by spectrophotometer. Then, the relationship between them was studied by gray relational analysis. Results: The results showed that the phlegm-phlegm function of the chloroform extract of Shaopian Beiqiu was the result of the synergistic effect of all the chemical constituents. Each characteristic peak represented the chemical composition and the order of contribution to the phlegm-phlegm function (numbering by characteristic peak) was 13> 13> 3> 8> 9, wherein 1, 3, 4, 10, 13, 16 The characteristic peaks were identified as iso-purpurin, psoralen, pricklyashin, Pd-Ib, chicago, peuformosin. CONCLUSION: The chloroform extract of Shaohibeiqinqianqiu has strong phlegm-activating activity, and there is a certain correspondence between HPLC fingerprints and phlegm-dampness.