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目的通过将孤雌胚胎干细胞(parthenogenetic embryonic stem cells,PgESCs)移植到新生鼠脑内,观察其增殖及向神经细胞方向分化的形态特点。方法分别向出生24h及72h小鼠脑内注射增强型绿色荧光蛋白(enhanced green fluorescence protein,EGFP)标记的孤雌胚胎干细胞,并于移植3d和7d后进行取材、观察。结果与对照组相比,出生24h和72h后进行干细胞移植的新生鼠中,移植3d后,孤雌胚胎干细胞可以稳定的在小鼠脑内生长,形态和分布无明显变化;7d后能够分化为具有神经细胞样突起的细胞团。结论 PgESCs具有胚胎干细胞相似的体内分化潜能,为中枢神经系统疾病的细胞替代治疗奠定理论基础。
Objective To observe the morphological characteristics of parthenogenetic embryonic stem cells (PGRESCs) transplanted into the brains of newborn rats and to observe their proliferation and differentiation toward neural cells. Methods Parthenogenetic embryonic stem cells labeled with enhanced green fluorescence protein (EGFP) were injected intracerebrally into the brains of mice born at 24h and 72h respectively. The cells were harvested and observed after 3d and 7d after transplantation. Results Compared with the control group, parthenogenetic embryonic stem cells could grow stably in mouse brain after 3 days of transplantation, and there was no obvious change in the morphology and distribution of newborn mice born after 24 hours and 72 hours after birth. After 7 days, they could differentiate into A cell mass with neuron-like projections. Conclusion PgESCs have similar in vivo differentiation potential of embryonic stem cells and lay a theoretical foundation for cell replacement therapy of central nervous system diseases.