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目的：进一步探讨新型抑癌基因ｐ１６与人类卵巢癌发生发展的关系。方法：采用Ｓ－Ｐ免疫组化方法对母系ＨＯ－８９１０细胞系、高转移ＨＯ－８９１０ＰＭ、ＮＳＭＯ模型和转移瘤以及６９例卵巢囊腺癌手术标本石蜡组织进行ｐ１６检测。结果：母系ＨＯ－８９１０细胞与高转移ＨＯ－８９１０ＰＭ细胞、ＮＳＭＯ模型及转移瘤均具有ｐ１６阳性表达，表达强度均以转移细胞为弱。６９例卵巢癌石蜡组织检测ｐ１６阳性５１例（占７３．９％），结合ｐ１６表达有无及强弱与病理组织学类型及分级、临床期别、腹水、癌肿播散、淋巴结转移及５年生存情况比较分析，发现随恶性程度、期别的增加，癌肿播散、淋巴结转移的出现，５年生存率的减低，ｐ１６表达明显减少，阳性强度减弱，具有统计学差异（Ｐ＜０．０１）。结论：ｐ１６基因不仅为细胞周期的固有调控者，亦与抑制肿瘤生长的关系密切，其缺失与表达水平的改变与卵巢癌的发生发展有关。
Objective: To further investigate the relationship between the novel tumor suppressor gene p16 and the development of human ovarian cancer. METHODS: p16 detection was performed on maternal HO-8910 cell line, high metastasis HO-8910PM, NSMO model and metastases, and 69 paraffin-embedded ovarian cystadenocarcinoma specimens using S-P immunohistochemistry. RESULTS: The maternal HO-8910 cells and highly metastatic HO-8910PM cells, NSMO model, and metastatic tumors all had p16 positive expression, and their expression intensity was weak in metastatic cells. Among the 69 cases of ovarian cancer, paraffin-embedded tissue was detected in 51 cases (73.9%), combined with the presence or absence of p16 expression, histological type and grade, clinical stage, ascites, disseminated cancer, lymph node metastasis, and 5 A comparative analysis of annual survival showed that with the increase of malignancy and stage, the occurrence of cancerous dissemination and lymph node metastasis, the 5-year survival rate was reduced, the expression of p16 was significantly reduced, and the positive intensity was weakened, with statistical significance (P<0). .01). Conclusion: The p16 gene is not only an innate regulator of the cell cycle, but also has a close relationship with the inhibition of tumor growth. The loss and expression levels of p16 gene are related to the occurrence and development of ovarian cancer.