论文部分内容阅读
目的观察反义血管内皮生长因子(anti-VEGF)原位表达对白血病细胞系K562在裸鼠体内生长的影响。方法选用白血病细胞系K562裸鼠体内接种成瘤后,肿瘤内注射重组反义VEGF真核表达质粒及空载体和PBS,检测反义VEGF体内转染情况及其对肿瘤生长的影响;免疫组化法比较实验组和对照组移植瘤微血管密度(MVD)及白血病细胞的凋亡情况。结果注射反义VEGF表达质粒能抑制肿瘤的生长,使肿瘤的MVD显著降低(25.6±5.77VS41±3.8,P<0.05),并且使肿瘤细胞凋亡增加。结论肿瘤内注射反义VEGF重组表达质粒在体内能有效转染肿瘤及其周围组织,反义VEGF体内的表达能抑制白血病细胞肿瘤的生长,降低肿瘤内MVD,促进白血病细胞的凋亡。反义VEGF基因治疗策略提供了一种新的白血病辅助治疗方案。
Objective To investigate the effect of anti-VEGF antisense VEGF (VEGF) expression on the growth of nude mice induced by leukemia cell line K562 in situ. Methods The leukemia cell line K562 was inoculated into the nude mice in vivo and then injected with recombinant antisense VEGF eukaryotic expression plasmid and empty vector and PBS to detect the transfection of antisense VEGF in vivo and its effect on tumor growth. Methods The microvessel density (MVD) and the apoptosis of leukemic cells in the experimental and control groups were compared. Results The antisense VEGF expression plasmid could inhibit tumor growth and significantly reduce the MVD (25.6 ± 5.77 vs41 ± 3.8, P <0.05) and increase the apoptosis of tumor cells. Conclusion The antisense VEGF recombinant plasmid can effectively transfect the tumor and its surrounding tissues in vivo. The antisense VEGF expression in vivo can inhibit the growth of leukemia cells, reduce the MVD in the tumor and promote the apoptosis of leukemia cells. Antisense VEGF gene therapy strategy provides a new leukemia adjuvant therapy.