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本研究采用豚鼠椭圆囊培养的方法观察了碱性成纤维细胞生长因子 (bFGF)对庆大霉素所致离体椭圆囊毛细胞毒性的拮抗作用。用庆大霉素造成培养椭圆囊毛细胞的损伤 ,实验组椭圆囊用含bFGF培养液培养 ,对照组培养液则不含bFGF。所有椭圆囊均行扫描电镜检查并行毛细胞计数。实验组毛细胞的存活数目显著高于对照组 (P <0 .0 5 )。bFGF对庆大霉素所致离体椭圆囊毛细胞毒性有拮抗作用。
In this study, we observed the antagonistic effect of basic fibroblast growth factor (bFGF) on the cytotoxicity of gentamicin-induced oval follicles in guinea pig oval sac culture. The injury of oval hair cells was induced by gentamicin. The oval sac of the experimental group was cultured with bFGF-containing culture medium, while the control group of the culture medium was free of bFGF. All oval sacs were examined by scanning electron microscopy parallel hair cell count. The number of hair cells in the experimental group was significantly higher than that in the control group (P <0.05). bFGF antagonizes gentamicin induced oocysts in vitro.