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小鼠胸腺细胞条件培养基可促进豚鼠骨髓造血祖细胞在体外琼脂和血浆凝块培养基中形成 CFU-M 和 CFU-C。培养基中种入1×10~4—2×10~5个骨髓有核细胞,在培养7天后 CFU-M 和 CFU-C 形成的数量与种入的骨髓细胞数之间呈线性关系。CFU-M 和 CFU-C 的祖细胞具有很高的辐射敏感性,骨髓细胞体外接受~(60)钴γ线照射后 CFU-M 的计数呈指数下降,其D_0值为92拉德与 CFU-C(D_0=88拉德)相近似。
Mouse thymocyte conditioned medium promotes the formation of CFU-M and CFU-C in guinea pig bone marrow hematopoietic progenitor cells in vitro in agar and plasma clot media. In the medium, 1 × 10 ~ 4-2 × 10 ~ 5 myeloid nucleated cells were planted, and the number of CFU-M and CFU-C formed after cultured for 7 days was linear with the number of marrow cells planted. The progenitor cells of CFU-M and CFU-C have high radiation sensitivity. The count of CFU-M decreased exponentially when the myeloid cells were irradiated with ~ (60) cobalt γ ray in vitro. The value of D 0 was 92 rad and CFU- C (D_0 = 88 rad) is similar.