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目的建立不同肥胖易感性大鼠动物模型,并探讨其能量代谢的特点。方法雄性SD大鼠60只,给予高脂饲料喂养3 w后,按体重增加量排序,上1/3(18只)作为肥胖易感(OP)组,下1/3(18只)作为肥胖抵抗(OR)组,中间1/3作为对照组。继续喂养12w后,每组随机挑选一半动物,每日给予半量饲料进行限食饲喂,另一半继续自由采食,持续2 w后,测量肥胖易感(OP)、肥胖抵抗(OR)及对照组大鼠不同状态下能量摄入量、基础代谢率(BMR)、静息代谢率(RMR)、基础能量消耗(BEE)等代谢指标。结果 OP大鼠体重、Lee’s指数、BMI指数、能量利用率均显著高于OR组和对照组,OR组与对照组之间无显著差异。在自由采食状态下,OP、OR和对照组间能量摄入水平无显著差异,OR组BMR、RMR及BEE水平显著高于对照组和OP组。在半量限食状态下,各组代谢水平较自由采食状态均有所降低,OR组BMR、RMR及BEE水平较自由采食状态降低幅度最大。结论 OP和OR大鼠在基础代谢方面存在差异,且OR较OP具有更为灵敏的能量平衡调节系统。
Objective To establish an animal model of different obesity susceptibility rats and to explore its energy metabolism characteristics. Methods Sixty male Sprague-Dawley rats were fed with high-fat diet for 3 weeks and then were sorted according to weight gain. One-third (18 rats) were used as obesity-susceptible (OP) Resistance (OR) group, the middle 1/3 as a control group. After 12 weeks of continuous feeding, half animals in each group were randomly selected, and half of the feed was given a limited feeding every day. The other half continued to eat freely for 2 weeks. Obese susceptibility (OP), obesity resistance (OR) and control The metabolic parameters such as energy intake, basal metabolic rate (BMR), resting metabolic rate (RMR) and basal energy expenditure (BEE) of rats in different groups were measured. Results The body weight, Lee’s index, BMI index and energy utilization rate of OP rats were significantly higher than those of OR group and control group. There was no significant difference between OR group and control group. There was no significant difference in energy intake between OP, OR and control groups in free-feeding state, while the levels of BMR, RMR and BEE in OR group were significantly higher than those in control group and OP group. In the semi-limited state, the metabolism of each group was lower than that of free-feeding, and the reduction of BMR, RMR and BEE in OR group was the largest than that of free-fed state. Conclusion OP and OR rats have differences in basal metabolism, and OR is more sensitive to energy balance regulation system than OP.