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黄酮是桑根皮中的重要活性成分,建立高效、便捷提取桑根皮总黄酮的工艺技术,有利于对桑根皮药用活性成分的深度开发。基于超声波辅助乙醇提取桑根皮总黄酮的单因素试验结果,进一步采用Box-Behnken中心组合设计结合响应面分析法优化提取工艺参数,选择提取时间、原料质量浓度和乙醇体积分数作为考察因子,以桑根皮总黄酮得率作为响应值,利用Design Expert软件对数据进行回归分析并结合验证试验结果,确定桑根皮总黄酮的最佳提取工艺条件为提取时间1.6 h、乙醇体积分数80%、原料质量浓度0.04 g/m L。在此工艺条件下提取总黄酮的得率为3.63%,与预测总黄酮得率(3.71%)的相对误差为2.16%,说明优化的工艺条件具有一定的实用价值。
Flavonoids are important active ingredients in mulberry root bark, and the establishment of an efficient and convenient extraction process of total flavonoids from mulberry root bark is beneficial to the further development of medicinal active ingredients of mulberry root bark. Based on the single-factor experiment results of extraction of total flavonoids from mulberry root bark by ultrasound-assisted ethanol extraction, process parameters were optimized by Box-Behnken combination design and response surface methodology. The extraction time, mass concentration of raw materials and ethanol volume fraction were selected as the investigation factors The results showed that the optimum extraction conditions of total flavonoids from mulberry root bark were 1.6 h extraction time, 80% ethanol volume fraction, Feedstock concentration 0.04 g / m L. The yield of total flavonoids extracted under this process was 3.63%, which was 2.16% compared with the predicted yield of total flavonoids (3.71%), which indicated that the optimized process conditions had some practical value.