论文部分内容阅读
目的和方法：采用间接免疫荧光法和支气管肺泡灌洗液（ｂｒｏｎｃｈｉａｌａｌｖｅｏｌａｒｌａｖａｇｅｆｌｕｉｄ，ＢＡＬＦ）技术观察１２例过敏性哮喘患者和２３例健康人外周血和ＢＡＬＦ中的Ｔ淋巴细胞亚群变化：结果：与健康对照组比较，过敏性哮喘患者外周血的Ｔ淋巴细胞亚群无明显改变，但其ＢＡＬＦ中的ＣＤ４＋细胞显著增高（５４．９７±４１４）％ｖｓ（７９．７１±９．６３）％，Ｐ＜００５；ＣＤ４＋与ＣＤ８＋细胞的比值也显著增高（１６４±０．３２ｖｓ２．３２±０．８３，Ｐ＜００５）。此外，过敏性哮喘患者ＢＡＬＦ中肥大细胞和嗜酸细胞百分比（００９±０．０４）％和（３６２±１．０６）％明显高于健康对照组（００２±０．０１）％和（０．３９±０．３０）％，Ｐ＜００５和Ｐ＜００１。结论：ＣＤ４＋细胞在哮喘的气道炎症中发挥重要作用。
PURPOSE AND METHODS: The changes of T lymphocyte subsets in peripheral blood and BALF of 12 patients with allergic asthma and 23 healthy individuals were observed by indirect immunofluorescence and bronchial asthma irrigation fluid (BALF) technique. Results: Compared with the control group, there was no significant change in T lymphocyte subsets in peripheral blood of patients with allergic asthma, but the CD4 + cells in BALF were significantly increased (54.97 ± 4.14% vs 79.71 ± 9.63% , P <005; the ratio of CD4 + to CD8 + cells was also significantly increased (164 ± 0.32vs2.32 ± 0.83, P <005). In addition, the percentages of mast cells and eosinophils in BALF were (009 ± 0.04)% and (362 ± 1.06)% in allergic asthma patients significantly higher than those in healthy controls (002 ± 0.01 )% And (0.39 ± 0.30)% respectively, P <005 and P <001. Conclusion: CD4 + cells play an important role in airway inflammation in asthma.