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本研究在兔胸主动脉环模型上观察了二苯三硝基苯肼（ＤＰＰＨ）自由基对乙酰胆碱（ＡＣｈ）诱导的血管内在依赖性舒张（ＥＤＲ）反应的影响。结果显示：０．２５μｍｏｌ·Ｌ－１ＤＰＰＨ与血管环孵育１０ｍｉｎ，显著抑制了ＡＣｈ诱导的血管ＥＤＲ反应。此种抑制作用为可逆性。拉米普利和缓激肽能显著保护ＤＰＰＨ损伤的血管ＥＤＲ反应。用ＤＰＰＨ建立自由基损伤模型的方法具有简单、方便、可靠、重复性好等优点。
In this study, we observed the effect of DPPH free radical on acetylcholine (ACh) -induced vascular endothelium-dependent relaxation (EDR) in a rabbit aortic ring model. The results showed that 0.25μmol·L-1DPPH incubated with the vascular ring for 10min significantly inhibited the ACh-induced vascular EDR response. This inhibitory effect is reversible. Lamipril and bradykinin significantly protect the vascular EDR response to DPPH injury. The method of establishing the free radical damage model with DPPH has the advantages of simple, convenient, reliable and good repeatability.