This study was conducted in selected sites of District Bahawalnagar namely, Shaheed Chowk, Chak40/F, Chak 163/7R and Fareedkot Tiba between March 2019 and August 2019. Sampling techniques included recording of burrows, flooding, burrow exaction, noose traps and pit fall traps were used to capture Uromastyx hardwickii. During this survey 150 individuals were captured from study area for morphological measurement and population estimation. The average snout to vent length (SVL) of male, female and juveniles were (172.50 ± 19.71) mm, (155.50 ± 18.72) mm and (86.25 ± 11.72) mm respectively. The average weight of male, female and juvenile was (93.06 ± 8.56) g, (88.16 ± 7.95) g and (40.16 ± 4.30) g respectively. The relative abundance (Pi) of males, females and juveniles were 0.53, 0.33 and 0.13. Indian spiny tailed lizard (U. hardwickii) has been listed in the IUCN Red list and CITES. Major threats to the species in the study area are increase in urbanization, habitat fragmentation, illegal hunting for meat and fat. In this regard strict implementation of the Wildlife act is needed to protect the wildlife of Punjab. Furthermore, comprehensive surveys are required in throughout Punjab to declare conservation status of U.hardwickii.