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目的探讨血清畸胎瘤细胞衍生生长因子(PCDGF)、血管内皮生长因子(VEGF)及胸苷激酶1(TK1)3项血清肿瘤指标对肺癌的诊断价值。方法选取2012年4月-2014年5月肺癌患者190例(A组)、同期门诊及住院部检查确诊的肺部良性病变患者100例(B组)、体检健康的志愿者60例(C组)。检测3组PCDGF、VEGF、TK1含量。采用酶联免疫吸附试验(ELISA)检测PCDGF、VEGF含量,用免疫印迹增强化学发光法检测TK1含量。统计3项指标诊断肺癌的敏感度、特异度以及准确性。并比较3项指标在肺癌不同病理分型、淋巴结转移、不同TNM分期的差异。结果 A组患者血清PCDGF、VEGF及TK1含量均显著高于B组和C组(P<0.05),B组和C组差异无统计学意义(P>0.05)。3项联合检测肺癌的灵敏度、特异度以及准确性均最高。VEGF在小细胞癌中表达最高,有淋巴转移的患者PCDGF、TK1含量均为高表达,在肺癌的不同分期中,PCDGF、TK1含量差异有统计学意义。结论血清PCDGF、VEGF、TK1含量对肺癌的诊断具有重要价值,联合诊断效果更优。
Objective To investigate the diagnostic value of three serum tumor markers of serum teratocarcinoma cell derived growth factor (PCDGF), vascular endothelial growth factor (VEGF) and thymidine kinase 1 (TK1) in lung cancer. Methods A total of 190 patients with lung cancer (A group) from April 2012 to May 2014 were selected, 100 patients with benign lung diseases diagnosed by outpatient and inpatient department during the same period (group B), 60 healthy volunteers (group C) ). The levels of PCDGF, VEGF and TK1 in three groups were detected. The contents of PCDGF and VEGF were detected by enzyme-linked immunosorbent assay (ELISA), and the content of TK1 was detected by immunoblotting with chemiluminescence. Statistics 3 indicators to diagnose lung cancer sensitivity, specificity and accuracy. The differences of the three indexes in different pathological types of lung cancer, lymph node metastasis and different TNM stages were compared. Results The serum levels of PCDGF, VEGF and TK1 in group A were significantly higher than those in group B and C (P <0.05). There was no significant difference between group B and group C (P> 0.05). The sensitivity, specificity and accuracy of the three joint detection of lung cancer were the highest. The expression of VEGF is the highest in small cell carcinoma. The expression of PCDGF and TK1 in patients with lymphatic metastasis are all highly expressed. There are significant differences in the expression of PCDGF and TK1 in different stages of lung cancer. Conclusion Serum levels of PCDGF, VEGF and TK1 are of great value in the diagnosis of lung cancer, and the combined diagnosis is more effective.