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为了探讨不同油菜品种对菌核病的抗病机制,以2个抗病品种中油821、德油5号和2个感病品种皖油14、杂优1号为材料,采用草酸浸叶以及不同致病力的核盘菌菌株NG4、WW2接种不同抗性的油菜品种后,对其叶片中丙二醛(MDA)、脯氨酸(Pro)、可溶性糖、可溶性蛋白含量的变化规律进行分析。结果表明,未接种时4个油菜品种MDA含量差异很小,接种发病后抗、感材料MDA含量均增加,但感病材料MDA含量明显高于抗病材料,且上升的幅度大。未接种时抗病材料Pro的含量高于感病材料,接种发病后Pro含量都上升,但抗病品种明显高于感病品种。可溶性糖含量都是先升高后降低,感病品种上升幅度显著高于抗病品种。接种后,抗性品种接种后24 h可溶性蛋白的含量达到最低值,48 h达最大值,后下降但高于对照。感病品种的含量24 h达到最大值,后降低。
In order to investigate the resistance mechanism of sclerotinia sclerotiorum to different sclerotinia sclerotiorum, two resistant varieties, Zhongyou 821, Deyou 5 and 2 susceptible varieties Wanyou 14 and Zaoyou 1 were used as materials, The pathogenicity of S. sclerotiorum NG4 and WW2 were analyzed by analyzing the changes of malondialdehyde (MDA), proline (Pro), soluble sugar and soluble protein in leaves of different rape cultivars. The results showed that there was little difference in the content of MDA between the four rapeseed cultivars without inoculation, and the contents of MDA in the resistant and susceptible cultivars increased after the inoculation, but the content of MDA in susceptible materials was significantly higher than that of the disease-resistant materials. The content of resistant material Pro in unvaccinated plants was higher than that of susceptible materials, and the content of Pro increased after inoculation, but the resistant varieties were significantly higher than the susceptible ones. Soluble sugar content increased first and then decreased, the susceptible varieties increased significantly higher than the resistant varieties. After inoculation, the content of soluble protein reached the minimum at 24 h after inoculation, reached the maximum at 48 h, then decreased but was higher than the control. The content of susceptible varieties reached the maximum at 24 h, then decreased.