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目的研究颈动脉粥样硬化微栓子阳性是否与基质金属蛋白酶-9(MMP-9)基因启动子C-1562T的多态性有相关性,为脑血管疾病的早期预防、诊断及治疗提供帮助。方法对被确诊为缺血性脑血管病的患者行颈动脉超声检查,筛选出患颈动脉粥样硬化不稳定斑块的患者120例,再行彩色经颅多普勒超声(TCD)监测微栓子,将其划分为微栓子阳性组63例与微栓子阴性组57例,用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对MMP-9基因启动子C-1562T位点进行基因分型,并随机抽取10%的样本对MMP-9基因启动子C-1562T的基因型进行直接测序证实。统计2组患者中C-1562T的基因型、等位基因频率,并分析MMP-9基因启动子C-1562T的多态性是否与微栓子阳性有相关性。结果微栓子阳性组CT型和TT型明显高于微栓子阴性组,2组比较差异有统计学意义(χ2=32.568,P<0.05);MMP-9的T等位基因是微栓子形成的危险因素(OR=10.000,P<0.05)。结论 MMP-9基因启动子C-1562T基因多态性与微栓子形成密切相关。
Objective To investigate whether the positive of carotid atherosclerosis micro-emboli is related to the polymorphism of matrix metalloproteinase-9 (MMP-9) gene promoter C-1562T and to help early prevention, diagnosis and treatment of cerebrovascular diseases . Methods A total of 120 patients with unstable plaque of carotid atherosclerotic plaque were screened by carotid ultrasonography in patients diagnosed as ischemic cerebrovascular disease. TCD microanalysis Emboli were divided into micro-emboli-positive group of 63 cases and micro-emboli-negative group of 57 cases, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology MMP-9 gene promoter C -1562T locus, and randomly selected 10% of the samples of MMP-9 gene promoter C-1562T genotypes confirmed by direct sequencing. The genotypes and allele frequencies of C-1562T in two groups of patients were analyzed. The polymorphism of C-1562T gene in MMP-9 gene was analyzed whether there was positive correlation between the polymorphism of C-1562T and micro-emboli. Results The CT-type and TT-type in the positive group were significantly higher than those in the negative group (P <0.05). The T-allele of MMP-9 was positive Risk factors for the formation (OR = 10.000, P <0.05). Conclusion The C-1562T gene polymorphism of MMP-9 gene promoter is closely related to the formation of microemboli.