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目的研究不同剂量维生素C(Vit C)对烧伤后延迟复苏大鼠血清肌钙蛋白Ⅰ(cTnⅠ)的影响,以了解Vit C对烧伤后延迟复苏心肌的保护作用。方法 40只Wistar大鼠随机分成4组:假手术组、延迟复苏组、Vit C低剂量组及Vit C高剂量组。复制烧伤模型30 h后,检测血清cTnⅠ、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性变化。结果使用Vit C可以降低烧伤后延迟复苏大鼠血清中cTnⅠ和MDA的含量、升高SOD活性(P<0.01);Vit C高剂量组降低烧伤后延迟复苏大鼠血清cTnⅠ和MDA的含量,升高SOD活性作用较Vit C低剂量组明显(P<0.05)。结论烧伤后延迟复苏时使用Vit C对心肌具有保护作用,大剂量Vit C的保护作用优于低剂量Vit C,其保护机制可能与抗氧自由基、抑制膜脂质过氧化有关。
Objective To investigate the effects of different doses of vitamin C on serum troponin Ⅰ (cTn Ⅰ) in rats with delayed resuscitation after burns to understand the protective effect of Vit C on delayed resuscitation myocardium after burn. Methods Forty Wistar rats were randomly divided into four groups: sham operation group, delayed resuscitation group, Vit C low dose group and Vit C high dose group. After 30 days, the content of cTnI, malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in serum were detected. Results Vit C could reduce the content of cTnⅠand MDA and increase the activity of SOD (P <0.01) in delayed resuscitation rats after burn. Vit C high dose group decreased the content of cTnⅠand MDA in the serum of delayed resuscitation rats after burn, High SOD activity than Vit C low dose group was significantly (P <0.05). Conclusions The use of Vit C for delayed resuscitation after burn injury has a protective effect on myocardium. The protective effect of high-dose Vit C is better than that of low-dose Vit C. The protective mechanism may be related to antioxidant free radicals and inhibition of membrane lipid peroxidation.