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目的分析登革 4型病毒 (DV4 )广东株的基因序列 ,比较其同源性 ,追查DV4的地理来源。方法RT -PCR扩增DV4NS2a -NS2b基因片段 ,克隆至PGEMT载体 ,分析其序列。结果1990年分离的 4个毒株在所分析的区段具有相同的序列 ,与DV4H2 4 1、814 6 6 9株、1978年广东株(GD785 6B2 )和 1993年广东株 (GZB5 )的核苷酸 (氨基酸 )同源性分别是 97% (98% )、94 % (96 % )、93% (98% )和 99% (98% )。GD785 6B2株与DV4H2 4 1、814 6 6 9株和 1993年广东株 (GZB5 )的核苷酸 (氨基酸 )同源性分别是 93% (98% )、96 % (97% )和 98% (93% )。结论 1990年引起广东省登革热流行的毒株很可能只有 1个 ,1990年的毒株与 1978年的毒株可能来自不同的疫源地。
Objective To analyze the gene sequence of Guangdong strain of dengue virus type 4 (DV4), compare its homology and trace the geographic origin of DV4. Methods The DV4NS2a-NS2b gene fragment was amplified by RT-PCR and cloned into PGEMT vector to analyze its sequence. Results The four isolates isolated in 1990 had the same sequence in the analyzed region. Compared with the nucleosides of DV4H2 4 1,814 6 6 9, Guangdong 1978 (GD785 6B2) and Guangdong 1993 (GZB5) The acid (amino acid) homologies were 97% (98%), 94% (96%), 93% (98%) and 99% (98%), respectively. The nucleotide (amino acid) homologies between the GD785 6B2 and DV4H2 4 1,814,669 strains and the 1993 Guangdong strain (GZB5) were 93% (98%), 96% (97%) and 98% (97% 93%). Conclusion It is possible that there is only one strain causing dengue in Guangdong Province in 1990. The strains in 1990 and 1978 may come from different foci.