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目的:通过检测宫颈癌前病变及宫颈癌患者宫颈分泌物HPV16,18的感染情况,探讨其与宫颈癌前病变及宫颈癌的相关性。方法:应用荧光定量PCR(FQ-PCR)技术对宫颈分泌物进行HPV16,18DNA定性检测。以光镜下病理学诊断作为CIN分级标准。CINⅡ及Ⅲ级是宫颈癌的癌前病变。结果:CINⅡ-Ⅲ级患者宫颈分泌物HPV16,18的检出率为67.7%。宫颈癌患者HPV16,18的检出率为91.8%。慢性宫颈炎患者的HPV16,18的检出率为28.6%。结论:HPV16,18感染与宫颈癌及宫颈癌前病变的发生发展密切相关。高危型HPV16,18DNA的检测对预防宫颈癌及宫颈癌的治疗有重要的指导作用。
Objective: To investigate the relationship between cervical precancerous lesions and cervical secretions HPV16,18 in patients with cervical precancerous lesions and cervical cancer, and to explore the correlation with cervical precancerous lesions and cervical cancer. Methods: Qualitative detection of HPV16 and 18 cervical secretions by fluorescence quantitative PCR (FQ-PCR) technique. Pathological diagnosis with light microscopy as CIN grading standards. CIN Ⅱ and Ⅲ grade cervical precancerous lesions. Results: The detection rate of cervical secretions HPV16,18 in CINⅡ-Ⅲ patients was 67.7%. Cervical cancer patients HPV16,18 detection rate was 91.8%. The detection rate of HPV16,18 in patients with chronic cervicitis was 28.6%. Conclusion: HPV16,18 infection is closely related to the occurrence and development of cervical cancer and cervical precancerous lesions. Detection of high-risk HPV16,18 DNA plays an important guiding role in the prevention of cervical cancer and cervical cancer.