论文部分内容阅读
目的采用临床分离的多重耐药鲍曼不动杆菌,建立慢性骨髓炎的小鼠动物模型。方法采用无菌或有细菌包裹的钢针插入小鼠胫骨平台干骺端。设立阴性对照组、金黄色葡萄球菌对照组和鲍曼不动杆菌实验组。结果苏木精-伊红(H&E)染色显示,2个细菌感染组均可见大量炎症细胞浸润,纤维增生,间质出血。革兰染色分别显示金黄色葡萄球菌对照组与鲍曼不动杆菌实验组有大量革兰阳性菌和革兰阴性菌。2个细菌感染组影像学检查均表现为胫骨周围软组织密度增高、骨膜增生。金黄色葡萄球菌对照组小鼠小腿胫骨针孔周围骨溶解和骨质破坏比鲍曼不动杆菌实验组更严重。结论本研究成功建立由多重耐药鲍曼不动杆菌引起的慢性骨髓炎模型,操作简便,成功率高,可用于今后骨髓炎的研究。
Objective To establish a mouse model of chronic osteomyelitis using clinically isolated multidrug-resistant Acinetobacter baumannii. Methods Sterile or bacterial-wrapped needles were inserted into the metaphysis of the tibial plateau of mice. A negative control group, Staphylococcus aureus control group and Acinetobacter baumannii experimental group were established. Results Hematoxylin and eosin (H & E) staining showed that a large number of inflammatory cell infiltration, fibrogenesis and interstitial hemorrhage were observed in both bacterial infection groups. Gram-stain showed a large number of Gram-positive bacteria and Gram-negative bacteria in Staphylococcus aureus control group and Acinetobacter baumannii experimental group respectively. Imaging imaging of the two bacterial infection groups showed increased soft tissue density around the tibia and periosteal hyperplasia. Staphylococcus aureus control group mouse calf tibial pinhole around osteolysis and bone destruction than Acinetobacter baumannii experimental group more serious. Conclusion This study successfully established a model of chronic osteomyelitis caused by multidrug-resistant Acinetobacter baumannii, with simple and convenient operation and high success rate. It can be used in the future research of osteomyelitis.