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通过体外实验，对Ｖｃ／ＦｅＳＯ４诱发的红细胞膜脂质过氧化（ＬＰ）发生条件及反应历程进行了探讨。结果表明：在３７℃水浴４５分钟内，红细胞膜蛋白浓度为０．７５ｍｇ／ｍｌ，５．０μｍｏｌ／ＬＦｅＳＯ４，ｐＨ值７．４的５ｍｍｏｌ／Ｌ磷酸盐缓冲液的条件下，较低浓度Ｖｃ（小于１．０ｍｍｏｌ／Ｌ）时可促进红细胞膜脂质过氧化过程，但高浓度Ｖｃ（大于１．０ｍｍｏｌ／Ｌ）则抑制红细胞膜脂质过氧化过程。Ｖｃ浓度为０．２５ｍｍｏｌ／Ｌ时，红细胞膜脂质过氧化产物丙二醛（ＭＤＡ）于反应后４５分钟内迅速上升，９０分钟接近峰值，以后趋于平衡。揭示：一定比例的Ｖｃ／ＦｅＳＯ４可有效地激发红细胞膜ＬＰ。本研究初步建立了红细胞膜ＬＰ模型。
Vc / FeSO4-induced erythrocyte membrane lipid peroxidation (LP) conditions and reaction process were discussed in vitro. The results showed that under the condition of 37 ℃ water bath for 45 minutes, the membrane protein concentration of erythrocytes was 0.75mg / ml, 5.0μmol / L FeSO4, 5mmol / L phosphate buffer with pH 7.4, the lower concentration Vc ( Less than 1.0mmol / L) could promote lipid peroxidation of erythrocyte membrane, but high concentration of Vc (greater than 1.0mmol / L) inhibited lipid peroxidation of erythrocyte membrane. Vc concentration of 0.25mmol / L, erythrocyte membrane lipid peroxidation product malondialdehyde (MDA) increased rapidly within 45 minutes after the reaction, 90 minutes close to the peak, then tends to balance. Revealed: a certain percentage of Vc / FeSO4 can effectively stimulate the erythrocyte membrane LP. This study initially established the LP membrane model of erythrocytes.