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将编码人免疫缺陷病毒I型 (HIV 1)核心蛋白p2 4的基因序列克隆到原核表达载体pET2 8(b)中 ,并转化不同的受体菌后 ,用IPTG诱导表达。SDS PAGE分析表明 ,受体菌BL2 1(DE3)plysS表达量最高 ,表达的重组p2 4蛋白占菌体总蛋白的 46 %。目的蛋白可与抗HIV 1p2 4单克隆抗体发生特异性反应。
The gene encoding human immunodeficiency virus type 1 (HIV 1) core protein p2 4 was cloned into the prokaryotic expression vector pET2 8 (b) and transformed into different recipient bacteria, then induced by IPTG. SDS PAGE analysis showed that the expression level of plysS was the highest in recipient strain BL2 1 (DE3), and the expressed recombinant p2 4 protein accounted for 46% of total bacterial proteins. The target protein can react specifically with anti-HIV 1p24 monoclonal antibody.