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目的研究L-精氨酸(LArg)、谷氨酰胺(Gln组)及两者合用(LArg+Gln)对保护创伤大鼠早期肝脏功能、扩张肝脏血管、缓解内毒素血症的作用。方法制作创伤大鼠模型,分为8、16、24和48h4个时段,每个时段分为分为正常对照组;NS组;LArg组;Gln组;LArg+Gln组等5组,每组9只大鼠。在各时相点,检测门静脉内毒素和肝组织NO2-/NO3-的含量。结果NO2-/NO3-含量:LArg组、LArg+Gln组NO2-/NO3-升高明显,与同时段正常对照组、NS组、Gln组比较有统计学意义(P<0.05)。NS组、Gln组肝组织中NO2-/NO3-轻度升高,与正常对照组比较差异有统计学意义(P<0.05);门静脉血浆内毒素的含量:NS组组门静脉血浆内毒素明显高于同时段其他组(P<0.01),LArg+Gln组门静脉内毒素含量较低,于同时段其他组比较差异有统计学意义(P<0.05)。结论在创伤大鼠早期,采用L精氨酸+谷氨酰胺对保护肝功能与缓解内毒素血症作用较佳。
Objective To investigate the effects of L-arginine (LArg), Gln (Gln) and LArg + Gln on the protection of early liver function, hepatic vasodilation and endotoxemia in traumatic rats. Methods Wound rat model was made and divided into 4 groups at 8, 16, 24 and 48h intervals, each group was divided into 5 groups: normal control group, NS group, LArg group, Gln group, LArg + Gln group, Only rats. At each time point, the contents of NO2- / NO3- in portal vein endotoxin and liver tissue were detected. Results NO2- / NO3- content: NO2- / NO3- increased significantly in LArg group and LArg + Gln group compared with normal control group, NS group and Gln group in the same period (P <0.05). The NO2- / NO3- increased slightly in NS group and Gln group compared with the normal control group (P <0.05). The content of endotoxin in the portal vein plasma was significantly higher in the NS group than in the NS group Compared with other groups in the same period (P <0.01), the content of portal vein endotoxin in LArg + Gln group was lower than that in other groups at the same time point (P <0.05). Conclusion In the early stage of traumatic rats, L-arginine + glutamine has better effect on protecting liver function and relieving endotoxemia.