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背景与目的乳腺癌患者的死亡原因几乎均为肿瘤远处转移。目前乳腺癌诊断尚无公认的特异性标志物,本文旨在探讨特异性的乳腺癌标志物─乳腺小粘蛋白(smallbreastepithelialmucin,SBEM)在乳腺癌外周血的表达及其意义。方法用巢式逆转录聚合酶链反应(Nested-RT-PCR)技术检测67例乳腺癌、16例乳腺良性肿瘤及20例健康志愿者外周静脉血中的SBEMmRNA的表达情况。结果SBEMmRNA在健康志愿者及乳腺良性肿瘤患者外周血中表达均为阴性;67例乳腺癌患者外周血中SBEMmRNA表达率为50.7%(34/67),在乳腺癌患者的临床分期Ⅰ、Ⅱ、Ⅲ和Ⅳ期中SBEMmRNA表达率分别为25.0%(2/8)、45.8%(11/24)、43.8%(7/16)和73.7%(14/19),在Ⅳ期乳腺癌患者外周血中SBEMmRNA的检出率明显高于其他各期(P<0.05)。SBEMmRNA的表达与患者年龄、原发癌灶大小、病理类型、ER和PR的状态无关(P>0.05)。结论SBEMmRNA特异表达于乳腺癌患者的外周血,有可能作为判断乳腺癌血道微小转移的指标之一。
Background and Objective The causes of death in breast cancer patients are almost distant metastasis of the tumor. At present, there is no recognized specific marker for breast cancer diagnosis. This article aims to investigate the expression and significance of specific breast cancer marker small Breastepithelialmucin (SBEM) in peripheral blood of breast cancer patients. Methods The expression of SBEM mRNA in peripheral blood of 67 cases of breast cancer, 16 cases of benign breast tumor and 20 healthy volunteers was detected by nested reverse transcriptase polymerase chain reaction (Nested-RT-PCR). Results SBEM mRNA expression was negative in peripheral blood of healthy volunteers and benign breast tumors. The expression of SBEM mRNA in peripheral blood of 67 patients with breast cancer was 50.7% (34/67). The clinical stage Ⅰ, Ⅱ, The expression rates of SBEM mRNA in stage Ⅲ and stage Ⅳ were 25.0% (2/8), 45.8% (11/24), 43.8% (7/16) and 73.7% (14/19) respectively. The detection rate of SBEM mRNA was significantly higher than other stages (P <0.05). The expression of SBEM mRNA was not related to the age of patients, the size of primary tumor, the pathological type, the state of ER and PR (P> 0.05). Conclusion SBEMmRNA is specifically expressed in peripheral blood of patients with breast cancer, which may be used as an indicator of micrometastasis in breast cancer.