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研制一种基于固定化铁氰化钾电子媒介体的介体型生化需氧量(BOD)生物电化学传感器芯片。基于微机电系统(MEMS)工艺制备金圆盘微电极,通过电化学循环伏安法在金圆盘微电极表面制备了聚吡咯和铁氰根离子复合膜,研究了该修饰电极的电化学性质及该固定化媒介体参与微生物降解有机物的电子传递能力。结果表明,固定于聚吡咯中的铁氰根离子作为电子媒介体具有较强的电子传输能力,能够代替溶解氧参与微生物对有机物的降解生化反应,从而实现BOD的快速检测。
A biodegradable biochemical electrochemical sensor chip based on immobilized potassium ferricyanide electronic media was developed. The gold disk microelectrode was prepared based on micro-electromechanical system (MEMS) process. The polypyrrole and iron cyanide ion composite membrane was prepared on the surface of gold disk microelectrode by electrochemical cyclic voltammetry. The electrochemical properties of the modified electrode And the immobilized mediator is involved in the electron transfer ability of the microorganism to degrade the organic substance. The results showed that the ferricyanide ions immobilized in polypyrrole have strong electron transport ability as electron mediator, which can replace dissolved oxygen to participate in the biodegradation of organic matter by microorganisms, so as to realize the rapid detection of BOD.