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利用RAPD和SSR技术对25°N野生大豆种群16个样本进行分子标记,从150多个RAPD引物中筛选出具有多态性的引物20个,共扩增出146个标记位点,其中具有多态性的有60个,占总位点的40.8％,平均遗传距离为0.1536,杂合度为0.3248。从60对SSR引物中筛选出14对具有多态性的引物,平均遗传距离为0.2209,杂合度为0.6961 聚类分析可将25°N野生大豆16个样本分成5类,表明种群内存在大量的遗传变异。为研究野生大豆种群内及种群间的遗传多态性探索了有效方法,并提出野生大豆核心种质资源的保存及取样对策。
Using RAPD and SSR techniques, 16 samples of 25 ° N wild soybean population were molecularly labeled. Twenty polymorphic primers were screened from more than 150 RAPD primers, and a total of 146 markers were amplified, of which there were more 60 were morphological, accounting for 40.8% of the total locus, the average genetic distance was 0.1536, the heterozygosity was 0.3248. Among the 60 pairs of SSR primers, 14 pairs of primers were screened with the average genetic distance of 0.2209 and the heterozygosity of 0.6961. Cluster analysis of 25 SSN wild soybeans could be divided into 5 categories, indicating that a large number of Genetic Variation. In order to study the genetic polymorphism of wild soybean populations and their populations, an effective method was explored and the conservation and sampling strategies of wild soybean core germplasm resources were proposed.