采用硼氢化钠还原法将试样中硒还原为挥发性硒化氢 ,氩为载气将硒化氢吹出并导入火焰原子化器进行原子荧光测定。以正交设计法对该还原反应、氢化物转移、原子化及原子荧光测定条件等进行了详细研究。实验结果表明 ,在佳化条件下 ,该法检出限为 0 .0 72μ g/ dm3,相对标准偏差为 2 .0 %。标准曲线表明 ,在 0 .0 0 0～ 1.0 0 0μ g/ dm3Se浓度范围内 ,有理想的线性关系。本法测定海洋生物样品硒的回收率为 95.8%～ 10 2 .4 %。 The sodium selenite in the sample was reduced to volatile hydrogen selenide by sodium borohydride reduction method, and hydrogen selenide was blown out and introduced into the flame atomizer for atomic fluorescence spectrometry with argon as the carrier gas. The reduction reaction, hydride transfer, atomization and atomic fluorescence measurement conditions were studied in detail by orthogonal design. The experimental results show that under the optimal conditions, the detection limit of this method is 0.72 μ g / dm3, with a relative standard deviation of 2.0%. The standard curve shows that there is an ideal linear relationship in the concentration range of 0. 0 0 ~ 1.0 0 0 μg / dm3Se. The recovery rate of selenium in marine samples by this method is 95.8% ~ 102.2%.