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目的观察大鼠肝细胞Ⅰ,Ⅲ型前胶原基因的表达及PDGF对其表达的影响.方法应用原位杂交技术检测分离培养的SD大鼠肝细胞(N＝30)内Ⅰ,Ⅲ型前胶原基因的表达.同时观察10μG/L(N＝30)和30μG/L(N＝30)PDGF促进前胶原基因表达的作用.测定基因表达颗粒总面积占细胞总面积的百分比,并作比较分析.结果无论正常肝细胞或是在两种浓度的PDGF存在时,肝细胞内均可见到Ⅰ,Ⅲ型前胶原基因的表达.正常肝细胞Ⅰ,Ⅲ型前胶原基因表达面积的百分比(％)为7?7±1?9和7?5±2?1;加10μG/LPDGF后为11?5±1?9和11?2±1?0,而加30μG/L后为15?2±3?4及18?1±2?8,且在后者中表达明显增强(P＜0?05及P＜0?01).结论PDGF在转录水平上促进肝细胞胶原的合成.“,”AIM To observe the expression of type Ⅰ and Ⅲ procollagen mRNAs of rat hepatocytes and effect of PDGF. METHODS The expression of type Ⅰ and Ⅲ procollagen mRNAs of rat hepatocytes and the effect of 10μg/L and 30μg/L PDGF ( n =30) were tested with in situ hybridization. The percentage of areas of mRNA expression in the total cell area was also determined. RESULTS The expression of type Ⅰ and Ⅲ procollagen mRNAs was seen in normal rat hepatocytes and with PDGF (two concentrations). The percen...