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本文用体内体外试验分析了17个9-菲甲醇类化合物对体外培养的恶性疟原虫及小鼠体内伯氏疟原虫的抗疟作用。体外试验:用对氯喹敏感对乙胺嘧啶有抗药性的Camp株和对氯喹、奎宁、乙胺嘧啶均有抗药性的Smith株恶性疟原虫作体外测定。化合物先用70%的酒精溶解到1mg/ml,再用RPMI_(1640)稀释。根据[~3H]次黄嘌呤的摄入量来衡量疟原虫的活性。培养液中[3~H]次黄嘌呤浓度为10μCi/ml。96井的微
In this paper, in vitro and in vivo experiments were conducted to analyze the anti-malarial effects of 17 9-phenanthroline compounds against Plasmodium falciparum and Plasmodium berghei in mice. In vitro tests: Camp strains resistant to pyrimethamine susceptible to chloroquine and Plasmodium falciparum resistant to chloroquine, quinine, and pyrimethamine were tested in vitro. Compounds were first solubilized to 1 mg / ml in 70% ethanol and then diluted in RPMI_ (1640). According to [~ 3H] hypoxanthine intake to measure the activity of malaria parasite. The culture solution [3 ~ H] hypoxanthine concentration of 10μCi / ml. Well 96 wells