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【目的】探讨植物生长调节剂乙烯利对橡胶树胶胞炭疽菌生长速率、孢子萌发和附着胞形成的影响,为橡胶树炭疽病防治提供新途径。【方法】以橡胶树胶胞炭疽菌RC169为供试菌株,利用菌丝生长速率法、孢子萌发法等常规植物病理学方法测定浓度为50.00、25.00、12.50、6.24、3.12和0 g/L的乙烯利处理对RC169菌丝生长的影响,以及浓度为0、0.2、0.4、0.8、1.6和3.2 g/L的乙烯利处理对RC169孢子萌发、附着胞形成等的影响。【结果】参试梯度浓度(50.00、25.00、12.50、6.24、3.12和0 g/L)乙烯利对RC169生长的抑菌率分别为98.6%、88.1%、34.7%、25.0%、8.1%和0,相应回归方程为y=2.9157x+1.9585,R2=0.9518;参试梯度浓度(0、0.2、0.4、0.8、1.6和3.2 g/L)乙烯利下RC169孢子萌发率分别为92.20%、36.70%、20.60%、12.30%、2.63%和0,相应线性回归方程为:y=-1.3945x+7.0663,R2=0.6803,RC169附着胞形成率分别为90.50%、5.37%、3.13%、1.10%、0和0,对应线性回归方程为:y=-1.7383x+6.7965,R2=0.8493。【结论】在一定浓度范围,乙烯利对橡胶树胶胞炭疽菌菌丝生长、孢子萌发、附着胞形成均具有抑制作用,且随着乙烯利浓度的升高,其抑制作用随之增强。
【Objective】 To investigate the effects of ethephon, a plant growth regulator, on the growth rate, spore germination and attachment formation of Colletotrichum gloeosporioides, providing a new approach for the prevention and control of rubber tree anthracnose. 【Method】 The rubbery Colletotrichum gloeosporioides RC169 was used as tested strain. The mycelial growth rate, spore germination and other conventional phytopathological methods were used to determine the contents of ethylene at concentrations of 50.00, 25.00, 12.50, 6.24, 3.12 and 0 g / L The effect of treatment on the mycelial growth of RC169 and the effect of ethephon at 0, 0.2, 0.4, 0.8, 1.6 and 3.2 g / L on spore germination and appressorial formation of RC169 were studied. 【Result】 The inhibitory rates of ethephon to RC169 at the concentrations of 50.00, 25.00, 12.50, 6.24, 3.12 and 0 g / L were 98.6%, 88.1%, 34.7%, 25.0%, 8.1% and 0% , The corresponding regression equation was y = 2.9157x + 1.9585, R2 = 0.9518. The spore germination rates of ethephon RC169 with gradient concentrations of 0, 0.2, 0.4, 0.8, 1.6 and 3.2 g / L were 92.20% and 36.70% , 20.60%, 12.30%, 2.63% and 0 respectively. The corresponding linear regression equations were: y = -1.3945x + 7.0663, R2 = 0.6803, and the formation rates of RC169 adherent cells were 90.50%, 5.37%, 3.13%, 1.10% And 0, the corresponding linear regression equation is: y = -1.7383x + 6.7965, R2 = 0.8493. 【Conclusion】 Etherelin inhibited the mycelial growth, spore germination and appressorium formation of Colletotrichum gloeosporioides in a certain range of concentration. With the increase of ethephon, ethephon inhibited the growth of colony.