大豆的细胞组织培养 ,在大豆遗传操作的研究中具有重要意义 ,受到国内外极大关注。现在已从大豆下胚轴、子叶、叶、顶芽和花药芽原生质体获得幼苗或植株的报道。但大豆的组织培养中与其他农作物相比在培养过程、成功率、植株再生方面仍较困难。本文对大豆的组织培养、植株再生、原生质体培养、花药培养、顶芽培养的研究进展和存在的问题作简要的评述 Soybean tissue culture, in soybean genetic operation research is of great significance, great concern at home and abroad. Seedlings or plants have now been reported from soybean hypocotyls, cotyledons, leaves, top buds and anther buds. However, compared with other crops, soybean tissue culture is still more difficult in terms of culture process, success rate and plant regeneration. In this paper, we briefly reviewed the research progress and existing problems of soybean tissue culture, plant regeneration, protoplast culture, anther culture and top bud culture