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目的介绍一种新的视网膜Ｍüｌｅｒ细胞条件化原代培养的方法。方法使用ＣｏｎＡ活化脾细胞培养上清液作为条件化培养基，分别培养人、大鼠、小鼠的视网膜Ｍüｌｅｒ细胞，并进行形态学观察及免疫组织化学染色。结果用该方法原代培养不同种类的视网膜Ｍüｌｅｒ细胞，免疫组织化学染色显示９０％以上的视网膜Ｍüｌｅｒ细胞的神经胶质纤维酸性蛋白（ｇｌｉａｌｆｉｂｒｉｌａｒｙａｃｉｄｉｃｐｒｏｔｅｉｎ，ＧＦＡＰ）和Ｓ－１００蛋白染色阳性。结论视网膜Ｍüｌｅｒ细胞的条件化原代培养是一种可靠的、快速获得不同种类视网膜Ｍüｌｅｒ细胞的理想方法。
Objective To introduce a new method of conditioned primary culture of retinal Müler cells. Methods ConA-activated splenocyte culture supernatants were used as conditioned medium. Human, rat and mouse retinal Müler cells were cultured and observed morphologically and immunohistochemically. Results Different types of retinal Müler cells were primary cultured by this method. Immunohistochemical staining showed that more than 90% of glial fibrillary acidic protein (glialfibrilary acid protein, GFAP) and S-100 protein were positive in retinal Müler cells. Conclusion The conditioned primary culture of retinal Müler cells is a reliable and rapid method for obtaining different types of retinal Müler cells.