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目的观察放射性舌炎病损局部病损组织血管内皮细胞损伤的动态变化,分析其与放射性舌炎病损程度的相关性。方法建立放射性舌炎SD大鼠动物模型;观察其放射性口腔黏膜炎指数(oral mucositis index,OMI);应用免疫组织化学方法(CD34)鉴定放射性舌炎病损局部病损组织血管内皮细胞;TUNEL检测RTG大鼠舌体病损组织内血管内皮细胞凋亡;统计学分析血管内皮细胞凋亡百分数与RTG口腔黏膜炎指数(OMI)间的时相变化关系。结果放射照射后3d可观察到明显的血管内皮细胞损伤和细胞凋亡,放射照射后的血管管腔变得明显不规则,管腔中可见有血栓形成,管腔内膜凹凸不平,血管扩张,管径变大,血管内皮细胞与基底膜分离脱落到管内,并可看到部分血管狭窄严重、闭塞。血管内皮细胞TUNEL检测到血管内皮细胞凋亡,5d组凋亡内皮细胞百分数(%)为(78.3±0.31),8d组为(89.3±0.83),14d组为(83.5±0.41),21d组为(69.3±0.57),28d组为(47.3±0.59)。5d、8d、14d、21d、28d时RTG口腔黏膜炎指数(OMI)与大鼠舌组织血管内皮细胞凋亡百分比的相关系数R=0.67(P=0.034)。结论RTG病损组织内血管内皮细胞损伤与RTG的病情相关,提示血管内皮细胞损伤可能在RTG病理过程中起作用并有潜在应用价值。
Objective To observe the dynamic changes of vascular endothelial cell injury in local lesions of radiation glossitis and analyze the correlation with the degree of radiation glossitis. Methods The animal model of radiation-induced glossitis was established. The oral mucositis index (OMI) was observed. The vascular endothelial cells were identified by immunohistochemistry (CD34) RTG rat tongue lesions tissue vascular endothelial cell apoptosis; statistical analysis of vascular endothelial cell apoptosis percentage and RTG oral mucositis index (OMI) the relationship between the phase changes. Results Significant vascular endothelial cell injury and apoptosis were observed 3d after irradiation. The vascular lumen became significantly irregular after irradiation. Thrombus was found in the lumen, and the lumen invagination was irregular and vasodilation was observed. Large diameter, vascular endothelial cells and basement membrane separation off into the tube, and can see some of the serious vascular stenosis, occlusion. The percentage of apoptotic endothelial cells in 5d group was (78.3 ± 0.31), that in 8d group was (89.3 ± 0.83), that in 14d group was (83.5 ± 0.41) and that in 21d group was (69.3 ± 0.57) and 28 (47.3 ± 0.59) respectively. The correlation coefficient (R = 0.67, P = 0.034) of RTG oral mucositis index (OMI) and percentage of apoptotic cells in rat tongue tissue at 5d, 8d, 14d, 21d and 28d. Conclusion The damage of vascular endothelial cells in RTG lesions is related to the condition of RTG, suggesting that vascular endothelial cell injury may play a role in the pathological process of RTG and has potential value.