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目的:探讨力竭运动后不同时相大鼠心肌Kir6.1基因表达的变化特点。方法:健康雄性SD大鼠80只,分为一次力竭游泳运动组和2周反复力竭游泳运动组及相应安静对照组。依据Thomas实验方案,建立不同程度的运动性心肌微损伤实验动物模型。力竭运动后,分时相取材,采用RT-PCR定量反转录方法分析心肌离子通道亚基Kir6.1在mRNA表达水平的变化。结果:(1)一次性力竭运动和反复力竭运动后24小时,心房肌中Kir6.1基因表达均显著下降(P<0.05)。(2)一次性力竭运动和反复力竭运动后4、12、24小时,心室肌中Kir6.1基因表达均显著升高(P<0.01)。结论:(1)力竭运动后,心室肌Kir6.1基因的表达水平总体呈上调变化,心房肌Kir6.1基因的表达水平在运动后24小时呈下调变化。(2)心室肌Kir6.1基因表达的上调对运动心肌有保护作用,而心房肌Kir6.1基因表达下调是心房肌易损伤的机制之一。
Objective: To investigate the changes of Kir6.1 gene expression in myocardium at different phases after exhaustive exercise. Methods: Eighty healthy male Sprague-Dawley rats were divided into two groups: one exhaustive swimming exercise group and two exhaustive exhaustive swimming exercise group and the corresponding quiet control group. According to the Thomas experimental protocol, experimental animal models of exercise-induced myocardial micro-injury were established. After exhaustive exercise, time-varying phase materials were collected and RT-PCR quantitative reverse transcription method was used to analyze the changes of myocardial ion channel subunit Kir6.1 in mRNA level. Results: (1) Kir6.1 gene expression in atrial myocardium decreased significantly (P <0.05) 24 hours after exhaustive exercise and exhaustive exercise. (2) Kir6.1 gene expression in ventricular muscle increased significantly (P <0.01) 4, 12 and 24 hours after exhaustive exercise and exhaustive exercise. Conclusion: (1) After exhaustive exercise, the expression level of Kir6.1 gene in the ventricular myocardium is generally up-regulated. The expression of Kir6.1 gene in atrial myocardium is down-regulated 24 hours after exercise. (2) The up-regulation of Kir6.1 gene expression in ventricular myocardium has protective effect on exercise-induced myocardium, and the down-regulation of Kir6.1 gene expression in atrial myocardium is one of the mechanisms of atrial injury.