论文部分内容阅读
目的:使用高剪切乳化机制备全氟化碳乳剂(PFCE),通过静脉注射PFCE对动脉粥样硬化(AS)模型大鼠进行干预,观察其内皮功能指标的变化,初步探讨PFCE对AS大鼠血管舒缩活性因子的调控作用及对内皮的可能保护作用。方法:将50只Wistar雄鼠随机分为5组:空白对照组,模型组,PFCE低、中、高剂量组,每组10只。除空白对照组用普通维持饲料外,其余均以维生素D3加高脂饲料喂养诱导实验性大鼠AS模型,PFCE低、中、高剂量组在造模同时给予不同剂量药物干预。12周后,测定各组大鼠血清NO、ET-1、TXA2、6-K-PG的含量,苏木精-伊红染色法观察各组大鼠主动脉病理变化。结果:模型组NO、TXA2含量显著降低,ET-1、6-K-PG含量显著升高,与空白对照组比较差异有统计学意义(P<0.05),且NO/ET-1比值、TXA2/6-K-PG比值平衡失调;PFCE高、中剂量组较模型组血清NO、TXA2含量显著升高,ET-1、6-K-PG含量显著降低(P<0.05),并改善NO/ET-1及TXA2/6-K-PG的比值(P<0.05);PFCE低剂量组与模型组相比血清中各指标差异无统计学意义(P>0.05);PFCE高剂量组与PFCE低剂量组相比血清NO、TXA2含量显著升高,ET-1、6-K-PG含量显著降低(P<0.05),与中等剂量组差异不显著(P>0.05);PFCE高、中剂量组较PFCE低剂量组减轻AS大鼠主动脉组织形态学变化更明显。结论:中等剂量以上PFCE可能通过调控AS大鼠血管舒缩活性因子,起到保护血管内皮的功能,发挥阻止AS进一步发生和发展的作用。
OBJECTIVE: To prepare perfluorocarbon emulsion (PFCE) by using high-shear emulsifier and to observe the change of endothelium function by injecting PFCE into the atherosclerotic (AS) model rats. To investigate the effect of PFCE on AS Regulation of rat vasomotor factors and its possible protective effect on the endothelium. Methods: Fifty Wistar male rats were randomly divided into 5 groups: control group, model group, PFCE low, medium and high dose group, 10 rats in each group. In addition to the blank control group with ordinary maintenance feed, the rest were fed with vitamin D3 and high fat diet induced experimental rat model of AS, PFCE low, medium and high dose group while giving different doses of drug intervention. After 12 weeks, the content of serum NO, ET-1, TXA2,6-K-PG in each group was determined. The pathological changes of the aorta in each group were observed by hematoxylin-eosin staining. Results: The levels of NO and TXA2 in model group were significantly lower than those in control group (P <0.05) / 6-K-PG ratio in the PFCE high and medium dose groups was significantly higher than that in the untreated group (P <0.05), and the NO / ET-1 and TXA2 / 6-K-PG ratio (P <0.05). There was no significant difference between the PFCE low dose group and the model group (P> 0.05) The levels of NO and TXA2 in serum were significantly higher than those in the middle dose group (P <0.05), while the levels of NO and TXA2 in serum were significantly increased (P <0.05) Compared with PFCE low-dose group, the morphological changes of aorta in AS rats were alleviated. CONCLUSION: PFCE at a moderate dose may play a role in protecting the vascular endothelium by regulating the vasomotor activity of AS rats, playing a role in preventing further occurrence and development of AS.