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染色体微切与微克隆技术由于可以在分子水平上对特定染色体区域进行基因定位和结构研究,因此,当Scalenghe首次在果蝇唾腺染色体上取得成功后,很快将这种技术应用于小鼠、人的染色体上.并利用该技术对染色体特定区域从分子水平上进行详细研究,并得到很多有价值的结果.我国对人类染色体微切与微克隆也已有报道,如:夏家辉等成功地构建了人类7号染色体专特性探针池和14个染色体区带特异性探针池。 但植物染色体的显微切割体外扩增与微克隆技术由于染色体同步化和制片困难与动物相比进展缓慢,目前只有在甜菜中与抗线虫有关染色体及Schondelmaier在大麦IHS染色体、Albani在小麦染色体上作过报道.我国则尚未开展这方面的研究。
Chromosome microchips and microcolonies Because of its ability to genetically position and structure specific chromosomal regions at the molecular level, this technique was soon applied to mice when Scalenghe was first successful on the Drosophila salivary gland chromosome , Human chromosome and using this technology to study the specific region of chromosome from the molecular level in detail and get a lot of valuable results.Chromosome microdissection and microcolonification have also been reported in our country such as Xia Jiahui successfully The human chromosome 7-specific probe pool and 14 chromosome-specific probe pools were constructed. However, in vitro amplification and microcoloning of plant chromosomes by microdissection are progressing slowly compared with animals due to chromosome synchronization and sheeting difficulties. At present, only the chromosomes associated with ant nematodes and Schondelmaier in the barley IHS chromosomes, Albani in wheat chromosomes Reported in our country has not yet carried out research in this area.