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了解丙肝和庚肝重叠感染的发生率,以及血浆和外周血单个核细胞中HCV-RNA及HGV-RNA的检出率是否有差异。方法:运用逆转录多聚酶链反应(RT-PCR)扩增的方法检测72例丙肝患者血浆、外周血单个核细胞中的HCV-RNA和HGV-RNA的情况。结果:在患者的血浆,单个核细胞中分别检出HGV-RNA阳性者11例和15例,两者同时呈阳性者10例。丙肝和庚肝重叠感染者在血浆中、在外用血单个核细胞中、和在血浆+外用血单个核细胞中、检出HCV-RNA和HGV-RNA的例数分别为9、11、6例,占总例数的12.4%、15.3%和8.3%。同步检测HCV-RNA和HGV-RNA感染率均是细胞高于血浆。结论:丙肝和庚肝重叠感染发生率与欧美、日本等国报道相似,同步检测对于避免临床漏诊和研究发病机理有重要意义。
To understand the incidence of hepatitis C and G over-infection, as well as the detection rate of HCV-RNA and HGV-RNA in plasma and peripheral blood mononuclear cells. Methods: HCV-RNA and HGV-RNA in plasma and peripheral blood mononuclear cells of 72 patients with hepatitis C were detected by reverse transcription-polymerase chain reaction (RT-PCR) amplification. Results: Eleven and 15 HGV-RNA positive cases were detected in the plasma and mononuclear cells of the patients, both of which were positive at the same time. HCV-RNA and HGV-RNA were detected in plasma, in peripheral blood mononuclear cells, and in plasma + peripheral blood mononuclear cells, respectively, in 9, 11, and 6 patients , Accounting for 12.4%, 15.3% and 8.3% of the total number of cases. Simultaneous detection of HCV-RNA and HGV-RNA infection rates were both higher in cells than in plasma. Conclusions: The prevalence of overlapping hepatitis C and GG infection is similar to that reported in Europe, the United States, Japan and other countries. Simultaneous detection is of great significance in avoiding clinical misdiagnosis and studying the pathogenesis.