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对于肿瘤转移机理和抗肿瘤转移药物研究来说,重要环节之一是准确而灵敏地检测肿瘤转移灶。本实验应用甲基丙烯酸甲酯和丁酯的混合物灌注小鼠肺脏,聚合后形成铸型,用扫描电子显微镜观察小鼠黑色素瘤B_(16)L_(10)肺转移灶,并经DJS—051微型计算机行电镜图象伪彩色处理,建立了一种较现有方法更灵敏和精确的新技术,以检测实验性肿瘤早期肺转移灶,取得较佳效果。正常肺泡铸型规则、饱满和光滑,肺泡间隙较均匀,与相邻组织界限清晰。肿瘤肺转移灶铸型向内塌陷,边界不清、肺泡间隙增大、肺泡损伤、变形或穿孔。图象伪彩色处理后,其识别能力更明显提高,而可分析较难辨认的肺转移灶。实验结果表明,铸型法得出的转移灶图象清晰,分辨率和灵敏度高,容易与标本处理中产生的假阳性现象区别,且能检测肺内早期转移灶,并作精确的定量分析,明显优于常规方法。这些特点使本方法有可能作为一种灵敏的抗肿瘤转移药物筛选的实验手段;亦有用于研究肿瘤转移机理。
One of the important aspects of tumor metastasis mechanism and anti-metastatic drug research is the accurate and sensitive detection of tumor metastases. In this experiment, mice lungs were perfused with a mixture of methyl methacrylate and butyl ester, and polymerized to form casts. Melanoma B_(16)L_(10) lung metastases were observed by scanning electron microscopy and DJS-051. The pseudo-color processing of micro-computer TEM images has established a new technique that is more sensitive and accurate than the existing methods to detect early lung metastases of experimental tumors and achieve better results. Normal alveolar casts are regular, plump, and smooth, and the alveolar space is relatively uniform, with clear boundaries to adjacent tissues. Tumor lung metastasis mold inward collapse, borders are unclear, alveolar space increases, alveolar damage, deformation or perforation. After the image is pseudo-colored, its recognition ability is significantly improved, and it is possible to analyze lung metastases that are difficult to identify. The experimental results show that the image of the metastatic foci obtained by the casting method is clear, with high resolution and sensitivity, it is easy to distinguish from the false positives produced during specimen processing, and early metastases in the lung can be detected and accurately quantified. Obviously better than conventional methods. These characteristics make this method possible as a sensitive anti-tumor drug screening experimental method; also used to study the mechanism of tumor metastasis.