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目的 从人脐血中分离纯化间充质干细胞(MSC) ,观察其移植对大鼠大脑中动脉栓塞后神经功能恢复的影响及细胞的存活、迁移向神经细胞分化的情况。方法 雄性SD大鼠45只,用线栓法建立大鼠大脑中动脉栓塞(MCAO)模型,大鼠随机分为3组:MSC移植组、单核细胞组和生理盐水组。移植后1、7、14、2 1、2 8d采用改良神经功能损害评分(mNSS)观察大鼠神经功能恢复情况,应用免疫组织化学和免疫荧光双标记技术检测5溴 2脱氧尿核苷(BrdU )标记的MSC细胞的存活、迁移及其胶质纤维酸性蛋白(GFAP)和神经元特异性核蛋白(NeuN)的表达。结果 人脐血MSC细胞移植可显著提高大鼠局灶性脑缺血后神经功能的恢复(P <0 .0 5 )。移植的MSC细胞可在大鼠脑组织中存活,并向缺血区域迁移,11.67%MSC细胞表达GFAP ,3 .72 %MSC细胞表达NeuN。结论 人脐血中含有MSC细胞并可促进局灶性脑缺血大鼠的神经功能恢复,移植细胞可在大鼠脑缺血区域中存活、迁移并向星形胶质细胞或神经元分化
OBJECTIVE: To isolate and purify mesenchymal stem cells (MSCs) from human umbilical cord blood (Umbilical cord blood) and observe their effects on the recovery of neurological function after middle cerebral artery occlusion and the survival and migration of the mesenchymal stem cells (MSCs) to neural cells. Methods Forty-five male Sprague-Dawley rats were randomly divided into 3 groups: MSCs transplantation group, mononuclear cells group and normal saline group. At 1, 7, 14, 2, and 12 days after transplantation, neurological functional recovery was observed by using modified neurological impairment score (mNSS). Immunohistochemistry and immunofluorescence double labeling were used to detect BrdU ) Labeled MSC cells, and the expression of glial fibrillary acidic protein (GFAP) and neuron specific nucleoprotein (NeuN). Results Human umbilical cord blood MSC cells transplantation can significantly improve the recovery of neurological function after focal cerebral ischemia in rats (P <0.05). Transplanted MSC cells could survive in rat brain tissue and migrate to the ischemic area, with 11.67% MSC expressing GFAP and 3.72% MSC expressing NeuN. Conclusion Human umbilical cord blood contains MSC cells and can promote the recovery of neurological function in focal cerebral ischemia rats. Transplanted cells can survive, migrate and differentiate into astrocytes or neurons