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观察睾酮对1,25-(OH)_2D_3抑制LoVo细胞增殖的影响及大肠癌患者血清睾酮水平的变化。方法:本研究采用细胞培养方法,观察1,25-(OH)_2D_3及睾酮(T)对培养的大肠癌LoVo细胞株增殖的影响,在此基础上,又观察了大肠癌患者血清T水平的变化。结果:1,25-(OH)_2D_3对LoVo细胞有明显的增殖抑制作用,这种作用具有浓度及时间依赖性,当11,25-(OH)_2D_3浓度达10~(-8)mol/L时,其抑制串可达30％。T单独作用时,对LoVo细胞的增殖无明显作用。但T与1,25-(OH)_2D_3合用时,可使后者对细胞增殖的抑制作用丧失。男性大肠癌患者较对照血清T水平明显降低(P<0.05),术后可回升接近正常水平。结论:T可拮抗1,25-(OH)_2D_3对LoVo细胞的增殖抑制效应,血清T水平可作为结直肠癌患者临床观测的指标之一。
Observe the effect of testosterone on the proliferation of LoVo cells with 1,25-(OH)2D_3 and the changes of serum testosterone levels in patients with colorectal cancer. Methods: In this study, cell culture method was used to observe the effects of 1,25-(OH)2D3 and testosterone (T) on the proliferation of cultured LoVo cell lines. On this basis, the serum T levels in patients with colorectal cancer were also observed. Variety. RESULTS: 1,25-(OH)_2D_3 had a significant inhibitory effect on the proliferation of LoVo cells. This effect was concentration- and time-dependent, when the concentration of 11,25-(OH)_2D_3 reached 10~(-8) mol/L. When it is, its suppression string can reach 30%. T alone had no effect on the proliferation of LoVo cells. However, when T is combined with 1,25-(OH)2D_3, the latter inhibits the inhibition of cell proliferation. Patients with colorectal cancer had significantly lower serum T levels than controls (P<0.05) and could recover to normal levels after surgery. Conclusion: T can antagonize the inhibitory effect of 1,25-(OH)_2D_3 on the proliferation of LoVo cells. Serum T level can be used as one of the indicators of clinical observation in patients with colorectal cancer.