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目的通过门静脉注射D-氨基半乳糖(DGal)和脂多糖的方法建立一种接近临床条件的猪急性肝功能衰竭(AHF)模型。方法22头健康家猪随机分为5组。组Ⅰ:门静脉注射生理盐水(3头);组Ⅱ:门静脉注射1μg/kg脂多糖(5例);组Ⅲ:门静脉注射0.5g/kgD-Gal(5例);组Ⅳ:门静脉注射0.5g/kgD-Gal+1μg/kg脂多糖(6例);组Ⅴ:门静脉注射0.5g/kgDGal+1μg/kg脂多糖,接受辅助性部分原位肝移植术(APOLT)(3例)。观察各组动物的临床表现、生化指标和病理改变。结果组Ⅰ和组Ⅱ动物全部存活,生化指标轻度改变。组Ⅲ动物1周内死亡率为40%(2/5)。组Ⅳ中5例(5/6,83%)在120h内死亡,注药后48h血清天冬氨酸氨基转移酶达最高峰(4912U/L±759U/L),同时伴有总胆红素、乳酸、血氨明显升高和凝血酶原时间延长。组Ⅴ动物行APOLT治疗后AHF逆转,第3天起各生化指标开始恢复,至第7天基本恢复正常,病理结果显示原肝得到部分再生。结论通过门静脉注射0.5g/kgDGal和1μg/kg脂多糖的方法可建立一种理想的猪急性肝功能衰竭模型。该模型可用于辅助性部分原位肝移植治疗急性肝功能衰竭的研究。
Objective To establish a near-clinical model of acute liver failure (AHF) in pigs by intravenous injection of D-galactosamine (DGal) and lipopolysaccharide (LPS). Methods 22 healthy domestic pigs were randomly divided into 5 groups. Group Ⅰ: portal vein injection of saline (3 heads); Group Ⅱ: portal vein injection of 1 μg / kg lipopolysaccharide (5 cases); Group Ⅲ: portal vein injection of 0.5g / kg D-Gal Group D: 0.5g / kgDGal + 1μg / kg lipopolysaccharide was injected into the portal vein to receive partial partial OLT (APOLT) (3 cases). The clinical manifestations, biochemical parameters and pathological changes of each group were observed. Results All animals in group Ⅰ and group Ⅱ survived with slight changes in biochemical parameters. Group 1 animals had a 1-week mortality rate of 40% (2/5). In group Ⅳ, 5 cases (5 / 6,83%) died within 120 hours. Serum aspartate aminotransferase (4912U / L ± 759U / L) peaked at 48 hours after injection, accompanied by total bilirubin , Lactic acid, blood ammonia was significantly elevated and prolonged prothrombin time. The animals in group Ⅴ underwent reversal of AHF after treatment with APOLT. The biochemical indexes began to recover on the third day and returned to normal on the seventh day. The pathological results showed that the original liver was partially regenerated. Conclusion An ideal model of acute liver failure in pigs can be established by injecting 0.5g / kgDGal and 1μg / kg lipopolysaccharide via portal vein. The model can be used for the study of adjuvant partial orthotopic liver transplantation in the treatment of acute liver failure.