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试验结果表明，以０．１％氯化汞消毒５～７ｍｉｎ并于消毒后１ｈ之内进行接种，茎顶转绿成活率可达９０％以上；茎顶的启动萌发率和萌发时间与外植体来源的母本材料年龄有关，取自试管无菌新梢和二年生幼苗的外植体启动萌发率显著高于取自六年生小树的外植体；黑暗培养有利于缩短启动萌发所需的时间，提高启动萌发率；试管新梢在转入生根培养基之前或转入生根培养基之后，先在黑暗条件下培养７天再转入间断光照下培养，能显著地提高生根率和根量；１／２ＭＳ培养基为较适宜的生根培养基，其ｐＨ值以５．４～６．４最佳，与蛭石加珍珠岩（１：１）或泥炭加珍珠岩（１：１）相比，以粗砂为基质更有利于生根试管苗的移栽成活与正常生长
The results showed that inoculation with 0.1% mercuric chloride for 5 ~ 7min and inoculation within 1h after disinfection, the survival rate of shoots was more than 90% Body-derived maternal material age-related, germination rates from explants of sterile shoots and biennial seedlings in vitro were significantly higher than those from young perennial trees; darkening was beneficial in shortening the time required to initiate germination Time, improve the germination rate; tube shoots into the rooting medium before or after switching to rooting medium, the first dark culture conditions and then transferred to the intermittent light culture, can significantly improve the rooting rate and root volume ; 1 / 2MS medium was more appropriate rooting medium, the pH value of 5.4 to 6.4 the best, and vermiculite plus perlite (1: 1) or peat plus perlite (1: 1) phase Compared with the coarse sand as the substrate is more conducive to the survival of rooting test tube seedlings and the normal growth