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采用 ABC 免疫酶标技术,观察了大鼠脾悬液和原位树状突细胞的 S-100蛋白免疫反应性。结果表明,脾悬液标本中的淋巴树状突细胞(LDC)和脾石蜡切片标本中的交错突细胞(IDC)、滤泡树状突细胞(FDC)均为显示棕黄色反应的 S-100蛋白阳性细胞。结合扫描电镜和透射电镜观察的结果,支持了 LDC 在体内的等同细胞是 IDC 的观点。
The S-100 protein immunoreactivity of rat spleen suspension and in situ dendritic cells was observed using ABC immunolabeling techniques. The results showed that the lymphoid dendritic cells (LDCs) in the spleen suspension specimens and the interdigitating cells (IDC) and follicular dendritic cells (FDC) in the spleen paraffin section specimens were all S-100s that showed a brown-yellow reaction. Protein-positive cells. Combining the results of scanning electron microscopy and transmission electron microscopy, it was supported by IDC’s view that the equivalent cell of LDC in vivo.