植物同工酶的发现,为分析鉴定植物品种开辟了一条新的途径。我们利用酯酶同工酶的电泳技术,对河南省商丘地区农林科学研究所用绿豆与甘薯进行无性嫁接获得的后代薯绿豆进行了分析,结果报告如下。一、材料选取:薯绿豆和小灰角绿豆(CK)两份材料,按照中科院遗传所杨太兴等制定的聚丙烯酰胺凝电泳法,采用发芽两天的幼苗植物制样,用10%的分离胶和4%的浓缩胶,电泳后用醋酸-α萘酯和快兰染色。二、结果讨论:如图所示,对照材料有E_1—E_6六条谱带。其中三条1级带;2条2级带;1条3级带。后代材料则有八条谱带。其中4条1级带;2条2级带;2条3级带,增加 The discovery of plant isozymes has opened up a new way for the analysis and identification of plant varieties. We use the esterase isozyme electrophoresis technology, Henan Shangqiu Agriculture and Forestry Science Institute of mung bean and sweet potato cloned offspring obtained offspring potato mung bean were analyzed, the results are reported as follows. First, the material selection: potato mung bean and small gray angle mung bean (CK) two materials, according to the Institute of Genetics Yang Taixing polyacrylamide gel electrophoresis developed by law, the use of two days of germination of plant samples, with 10% And 4% concentrated gel, after electrophoresis with α-naphthyl acetate and fast blue stain. Second, the results of the discussion: As shown, the control material E_1-E_6 six bands. One of three 1-band; 2 2-band; 1 3-band. There are eight bands of descendant materials. Of which four 1-band; 2 2-band; 2 3-band, increase