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目的 探讨苯乙烯的遗传毒性机制。方法 采用碱性单细胞凝胶电泳(SCGE)方法,分离健康人外周血淋巴细胞,在体外用苯乙烯或其中间代谢产物7,8 氧化苯乙烯(SO)染毒1 h后,测量彗星尾长。结果 苯乙烯0. 100、0. 200、0. 400 mmol/L染毒组的彗星尾长分别为(12. 06±5. 82)、(9.25±3.24)、(8.60±4.30)μm,与空白及溶剂对照组[(6.69±2.17)、(6.56±0.96)μm]的差异有统计学意义(P<0.05)。SO 0.050、0.100、0.200、0.400 mmol/L组彗星尾长分别为(11.35±4.32)、(15.05±4.11)、(20.10±2.11)、(26.64±2.16)μm,与空白及溶剂对照组[(7.20±2.85)、(6.95±1.88)μm]的差异均具有统计学意义(P<0.05),并呈现出明显的剂量-反应关系(r=0.97,P=0.006)。结论 苯乙烯及SO均可致人淋巴细胞DNA的断裂,SO的作用强于苯乙烯。
Objective To investigate the genotoxic mechanism of styrene. Methods Peripheral blood lymphocytes were isolated by alkaline single-cell gel electrophoresis (SCGE) and stained with styrene or its intermediate metabolites 7 and 8 (SO) for 1 h. long. Results The tail lengths of comets in the groups of 0. 100,0. 200,0. 400 mmol / L styrene were (12.06 ± 5.82), (9.25 ± 3.24) and (8.60 ± 4.30) μm, respectively, There was significant difference between blank control group and solvent control group [(6.69 ± 2.17), (6.56 ± 0.96) μm] (P <0.05). The tail lengths of comets in SO 0.050,0.100,0.200,0.400 mmol / L group were (11.35 ± 4.32), (15.05 ± 4.11), (20.10 ± 2.11) and (26.64 ± 2.16) μm, respectively, 7.20 ± 2.85, 6.95 ± 1.88 μm, respectively) (P <0.05), and showed a dose-response relationship (r = 0.97, P = 0.006). Conclusion Both styrene and SO can cause the cleavage of DNA in human lymphocytes, and the effect of SO is stronger than that of styrene.