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目的建立HPLC法同时测定丹芎通脉颗粒中丹酚酸B、阿魏酸、羟基红花黄色素A、没食子酸和咖啡酸的方法。方法采用Thermo ODS-2 Hypersil(250 mm×4.6 mm,5μm)色谱柱,以0.1%乙酸水溶液-甲醇为流动相,梯度洗脱,体积流量1.0 mL/min,检测波长为286 nm,柱温30℃。结果丹芎通脉颗粒中丹酚酸B、阿魏酸、羟基红花黄色素A、没食子酸和咖啡酸分别在3.18~50.88μg/mL(r=0.999 0)、2.00~32.00μg/mL(r=0.999 5)、6.00~96.00(r=0.999 6)、0.60~9.60μg/mL(r=0.999 4)和1.00~16.00μg/mL(r=0.999 5)与峰面积呈良好线性关系;平均回收率分别98.12%、97.23%、98.51%、99.11%和97.01%(n=6)。结论该方法准确可靠,简单、快速,重复性好,可用于丹芎通脉颗粒中丹酚酸B、羟基红花黄色素A、阿魏酸、没食子酸和咖啡酸成分的质量控制。
OBJECTIVE To establish a HPLC method for the simultaneous determination of salvianolic acid B, ferulic acid, hydroxysafflor yellow A, gallic acid and caffeic acid in Danxiong Tongmai granules. Methods The column was eluted with a gradient of 0.1% acetic acid in methanol with a flow rate of 1.0 mL / min on a Thermo ODS-2 Hypersil (250 mm × 4.6 mm, 5 μm) column. The detection wavelength was 286 nm and the column temperature was 30 ℃. Results The contents of salvianolic acid B, ferulic acid, hydroxysafflor yellow A, gallic acid and caffeic acid in Danxiong Tongmai Granule were 3.18 ~ 50.88μg / mL (r = 0.999 0) and 2.00 ~ 32.00μg / mL (r = 0.999 5), 6.00-96.00 (r = 0.999 6), 0.60-9.60 μg / mL (r = 0.999 4) and 1.00-16.00 μg / mL (r = 0.999 5) showed a good linear relationship with the peak area. The recoveries were 98.12%, 97.23%, 98.51%, 99.11% and 97.01%, respectively (n = 6). Conclusion The method is accurate, reliable, simple, rapid and reproducible. It can be used for the quality control of salvianolic acid B, hydroxysafflor yellow A, ferulic acid, gallic acid and caffeic acid in Danxiong Tongmai Granules.