论文部分内容阅读
目的:建立三七叶总皂苷中人参皂苷Rb_3的提取方法及含量测定方法。方法:采用乙醇回流提取法、大孔树脂吸附法、离子交换法等提取三七叶总皂苷,HPLC法对三七叶总皂苷中人参皂苷Rb_3进行定量分析,研究其质量标准。结果:三七叶总皂苷产率为12%,人参皂苷Rb3的线性范围为(1.078~6.468)×10~(-3) mg,回归方程为Y=2.2717X+0.8629,r=0.9998;RSD为0.944%。人参皂苷Rb_3的含量为11.17%>10%(2010年版《中国药典》规定≥10%)。结论:本研究所用方法对三七叶总皂苷的提取率高,方法简单可行;采用HPLC检测其含量,专属性强,重现性好,准确度高。
Objective: To establish a method for the determination of ginsenoside Rb_3 in Panax notoginseng leaf and its content determination. Methods: The total saponins of Rhizoma Panax notoginsee were extracted by ethanol reflux extraction, macroporous resin adsorption method and ion exchange method. The quantitative analysis of ginsenoside Rb_3 in Panax notoginseng saponins by HPLC was carried out to study the quality standard. Results: The yield of Panax notoginseng was 12%. The linear range of ginsenoside Rb3 was (1.078 ~ 6.468) × 10 ~ (-3) mg. The regression equation was Y = 2.2717X + 0.8629, r = 0.9998. 0.944%. Ginsenoside Rb_3 content of 11.17%> 10% (2010 edition of the “Chinese Pharmacopoeia” ≥ 10%). Conclusion: The method used in this study has high extraction rate of total saponins from Panax notoginseng, the method is simple and feasible. The content of the total saponins from Panax notoginseng leaves is high, specificity and repeatability are high and the accuracy is high.