以鬼臼毒素为原料合成了几种衍生物 ,用质谱和核磁共振鉴定了其结构。以5龄菜青虫 (Pieris rapae)、3龄小菜蛾 (Plutella xylostella)和 3龄粘虫 (Mythimna separataWalker)为试虫 ,测定了其生物活性。结果表明 ,鬼臼毒素类似物对菜青虫表现出明显的结构与活性规律。母体的几种异构体中 ,鬼臼毒素的活性最强 ;4′位为甲氧基的衍生物较 4’位为羟基的活性要高 ;内酯环打开的衍生物活性降低。 Several derivatives were synthesized from podophyllotoxin and identified by mass spectrometry and nuclear magnetic resonance. The biological activity of Pieris rapae, Plutella xylostella, 3rd instar Mythimna separata Walker was tested. The results showed that podophyllotoxin analogues showed obvious structure and activity on Pieris rapae. Podophyllotoxin was the most potent among several isomers of the parent; the methoxy derivative at the 4 ’position was more active than the hydroxyl at the 4’ position; the activity of the lactone ring opened derivative was reduced.